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Bioinformatics and DNA-extraction strategies to reliably

 · Archived formalin fixed paraffin embedded (FFPE) samples are valuable clinical resources to examine clinically relevant morphology features and also to study genetic changes. However DNA quality and quantity of FFPE samples are often sub-optimal and resulting NGS-based genetics variant detections are prone to false positives. Evaluations of wet-lab and bioinformatics approaches are

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A fully automatable enzymatic method for DNA extraction

 · Background DNA extraction from plant tissues unlike DNA isolation from mammalian tissues remains difficult due to the presence of a rigid cell wall around the plant cells. Currently used methods inevitably require a laborious mechanical grinding step necessary to disrupt the cell wall for the release of DNA. Results Using a cocktail of different carbohydrases a method was developed that

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Analysis of protein glycosylation by mass spectrometry

 · Unité Mixte de Recherche CNRS/USTL 8576 Glycobiologie Structurale et Fonctionnelle IFR 147 Bâtiment C9 Université des Sciences et Technologies de Lille 1 Villeneuve d

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PureLink™ RNA Mini KitThermo Fisher Scientific

The PureLink RNA Mini Kit is a column-based kit used to isolate high-quality total RNA from a wide variety of sample types in 20 minutes using standard laboratory equipment. The kit includes RNase-free lysis and wash solutions that protect RNA from RNases while liberating the RNA from DNA proteins

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LuminexA DiaSorin Company Complexity Simplified

Luminex Corporation develops manufactures and markets biological testing technologies in the clinical diagnostic and life science industries.

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Deep eutectic solvents (DESs) for cellulose dissolution a

 · Deep eutectic solvents (DESs) which are a novel class of sustainable designer solvents have attracted considerable attentions in the field of cellulose chemistry. Due to their low cost and analogous physico-chemical properties to ionic liquids DESs are expected to be alternative solvents for dissolving cellulose. However at present the solubility of cellulose in DESs is much lower than in

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A Short Review of Techniques for Phenol Removal from

 · Guan X He D Ma J Chen G. Application of permanganate in the oxidation of micropollutants a mini review. Front Environ Sci Eng China. 20104(4) 405–13. CAS Article Google Scholar 35. Jiang JQ Durai HB Winzenbacher R Petri M Seitz W. Drinking water treatment by in situ generated ferrate (VI).

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A single protocol for extraction of gDNA from bacteria and

 · LiOAC-SDS gDNA extraction .gg and 1 µL of the supernatant was used for PCR. QiAamp DNA Mini Kit. The manufacturer s recommendations were followed for all organisms that included initial treatment in ATL buffer either without pretreatment (Gram-negative bacteria) or after treatment with lysostaphin (Gram-positive bacteria) or lyticase (yeast

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MÉMOIRE DE FIN D ÉTUDES présenté pour l obtention du

 · Au sein de l Institut National de la Recherche Agronomique (INRA) Unité Mixte de Recherche (UMR) Innovation et dans le cadre du projet de recherche en partenariat pour et sur le développement régional (PSDR) « Coxinel » lancé en 2008 un stage de fin d étude de 6 mois a été effectué. Il est

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Extraction and recovery of 2-butoxyethanol from aqueous

 · It was determined that D-limonene and 1-butoxy-2-propanol make up 31.8 ± 0.019 and 18.7 ± 0.0361 wt (n = 3) of Revert Flow respectively. As shown in Fig. 5 extraction efficiency of D-limonene and 1-butoxy-2-propanol from Revert Flow were also determined. After 5 washes with methylene chloride 94.5 overall efficiency was obtained for D

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SILEX a fast and inexpensive high-quality DNA extraction

 · The use of sequencing and genotyping platforms has undergone dramatic improvements enabling the generation of a wealth of genomic information. Despite this progress the availability of high-quality genomic DNA (gDNA) in sufficient concentrations is often a main limitation especially for third-generation sequencing platforms. A variety of DNA extraction methods and commercial kits are

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Critical points of DNA quantification by real-time PCR

 · Real-time PCR is the technique of choice for nucleic acid quantification. In the field of detection of genetically modified organisms (GMOs) quantification of biotech products may be required to fulfil legislative requirements. However successful quantification depends crucially on the quality of the sample DNA analyzed. Methods for GMO detection are generally validated on certified reference

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Removal of HFPO-DA (GenX) from aqueous solutions A

With regard to HFPO-DA the first published work on removal by membrane was carried out by Pica et al. 78 in 2019 who utilized an NF membrane (NF90) to treat semiconductor industry effluent. The applied membrane was able to eliminate 99.5 of HFPO-DA from contaminated water in the presence of N a 2 S O 4 and NaCl.

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QIAquick Gel Extraction KitQiagen

 · The QIAquick Gel Extraction Kit provides spin columns buffers and collection tubes for silica-membrane-based purification of DNA fragments from gels (up to 400 mg slices) or enzymatic reactions. DNA ranging from 70 bp to 10 kb is purified using a simple and fast bind-wash-elute procedure and an elution volume of 30–50 μl.

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Massively parallel de novo protein design for targeted

 · De novo protein design holds promise for creating small stable proteins with shapes customized to bind therapeutic targets. We describe a massively

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Gold Processing Extraction Smelting Plant Design

Gold ore. Prominer maintains a team of senior gold processing engineers with expertise and global experience. These gold professionals are specifically in gold processing through various beneficiation technologies for gold ore of different characteristics such as flotation cyanide leaching gravity separation etc. to achieve the processing plant of optimal and cost-efficient process designs.

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Evaluation of the impact of six different DNA extraction

 · De Lipthay JR Enzinger C Johnsen K Aamand J Sørensen SJ (2004) Impact of DNA extraction method on bacterial community composition measured by denaturing gradient gel electrophoresis. Soil Biol Biochem 36(10) 1607–1614. doi 10.1016/j.soilbio.2004.03.011

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How to Use Feature Extraction on Tabular Data for

 · Machine learning predictive modeling performance is only as good as your data and your data is only as good as the way you prepare it for modeling. The most common approach to data preparation is to study a dataset and review the expectations of a machine learning algorithm then carefully choose the most appropriate data preparation techniques to transform the raw

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SiliCycle Inc. UltraPure Silica Gels and Chemistry

Founded in 1995 SiliCycle ® Inc. is a worldwide leader in the development the manufacturing and the commercialization of high value silica-based and specialty products for chromatography analytical and organic chemistry.

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Integrated Devices for Non‐Invasive DiagnosticsAtes

 · The versatility of CRISPR-associated (Cas) enzymes enables i) to target a variety of nucleic acids (i.e. double-stranded DNAs or single-stranded RNAs or DNAs) ii) to work under different cleavage conditions (temperature or buffer) and ii) to combine analytical procedures such as sample extraction amplification and analysis in a single step.

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Dna extraction Sigma-Aldrich

The yeast heterotetrameric GINS complex is made up of Sld5 (GINS4 MIM 610611) Psf1 (GINS1 MIM 610608) Psf2 and Psf3 (GINS3 MIM 610610). The formation of this complex is essential for the initiation of DNA replication in yeast and Xenopus egg extracts (Ueno et al. 2005 PubMed

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D8-MMAE (D8-Monomethyl auristatin E) Deuterated

D8-MMAE (D8-Monomethyl auristatin E) is a deuterated labeled MMAE a potent mitotic inhibitor and a tubulin inhibitor. Antibody-drug conjugates (ADC) comprise targeting antibodies armed with potent small-molecule payloads. ADCs are generated to target different receptors on the anaplastic large cell lymphoma line L-82 but delivered the same

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Isolation of antimicrobial peptides from different plant

 · Plants are good sources of biologically active compounds with antimicrobial activity including polypeptides. Antimicrobial peptides (AMPs) represent one of the main barriers of plant innate immunity to environmental stress factors and are attracting much research interest. There are some extraction methods for isolation of AMPs from plant organs based on the type of extractant and initial

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3D of Personalized Thick and Perfusable Cardiac

 · d) Cell viability before and after and after extraction. e) 3D confocal image of a double layered construct printed in the support medium. f) A single strand of the personalized hydrogel within the support. g h) Accurate high resolution thick structures printed from the personalized hydrogel.

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BASFUnited States

BASF co-founded a global alliance of nearly 30 companies to advance solutions that reduce and eliminate plastic waste in the environment especially in the ocean. Find out more Investor Relations Visit our website for information on the BASF share and an overview of the BASF Group. Investor Relations

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A clinician s guide to microbiome analysis Nature

 · Zerbino D. R. Birney E. Velvet algorithms for de novo short read assembly using de Bruijn graphs. Genome Res. 18 821–829 (2008). Article CAS PubMed PubMed Central Google Scholar

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Products Applied Medical

Contained Extraction System . Epix Laparoscopic Instrumentation . Epix Universal Clip Applier . GelPOINT Path Transanal Procedural Pack . Inzii Retrieval Systems . Simsei Laparoscopic Training Systems . Silhouette Ureteral Stents .

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Frontiers Effects of Chemical and Mechanical Lysis on

 · QIAamp Fast DNA Stool Mini Kit (QIAGEN Valencia CA United States) and RBB C (Yu and Morrison 2004) methodologies are widely employed to extract microbial DNA from rumen samples and can exhibit different efficiencies of obtaining DNA yield quality and downstream amplicon sequence analysis. No study has conducted to investigate the contributions of chemical and mechanical lysis on

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Integrated Devices for Non‐Invasive DiagnosticsAtes

 · The versatility of CRISPR-associated (Cas) enzymes enables i) to target a variety of nucleic acids (i.e. double-stranded DNAs or single-stranded RNAs or DNAs) ii) to work under different cleavage conditions (temperature or buffer) and ii) to combine analytical procedures such as sample extraction amplification and analysis in a single step.

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Dynamic-ETL a hybrid approach for health data extraction

 · The accuracy and reliability of the D-ETL rules and the D-ETL engine rely on the accuracy and reliability of content of the D-ETL rules. Additional technical performance metrics to be examined in a larger scale implementation would include aspects of improved data quality including accuracy (e.g. fewer errors in mapping) and completeness (e.g

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QIAamp DSP DNA Blood Mini KitQiagen

 · The QIAamp DSP DNA Blood Mini Kit uses well-established QIAamp technology for purifying genomic DNA. The QIAamp silica-based membrane specifically binds DNA in the lysed sample while the rest of the lysate is rapidly removed by either centrifugation or vacuum. The bound DNA is efficiently washed to remove contaminants and then eluted in

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Hydrogel particles improve detection of SARS-CoV-2 RNA

 · Here we present a rapid and versatile method for capturing and concentrating SARS-CoV-2 from contrived transport medium and saliva samples using

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Method for improving the quality of genomic DNA

 · Although genomic DNA isolation using the Chelex 100 resin is rapid and inexpensive the DNA obtained by this method has a low concentration in solution and contains suspended impurities. The presence of debris in the DNA solution may result in degradation of DNA on long term storage and inhibition of the polymerase chain reaction. In order to remove impurities and concentrate the DNA in

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Isolation of antimicrobial peptides from different plant

 · Plants are good sources of biologically active compounds with antimicrobial activity including polypeptides. Antimicrobial peptides (AMPs) represent one of the main barriers of plant innate immunity to environmental stress factors and are attracting much research interest. There are some extraction methods for isolation of AMPs from plant organs based on the type of extractant and initial

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Investigation of the biological functions of heparan

Heparan sulfate (HS) is a highly sulfated polysaccharide playing essential physiological and pathophysiological roles in the animal kingdom. Heparin a highly sulfated form of HS is a widely used anticoagulant drug. Isolated from biological sources both heparin and HS are polysaccharide mixtures with diffe RSC Chemical Biology Transparent Peer Review Collection

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The sugar‐responsive circadian clock regulator bZIP63

 · Extraction and quantification of starch. Starch was determined in the insoluble material after ethanolic extraction of soluble sugars followed by enzymatic digestion with α-amyloglucosidase and α-amylase (Hendriks et al. 2003). Whole rosettes from six 30-d-old plants were used as a single sample.

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PureLink™ Quick Gel Extraction Kit

The PureLink Quick Gel Extraction Kit allows you to rapidly and efficiently purify DNA fragments from TAE or TBE agarose gels of various percentages. DNA can be extracted and purified from agarose gels with different melting points in 30 minutes using PureLink silica membrane-based quick gel extrac

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